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    • 专利摘要:
    Electrode device for the detection of simple and portable lactic acid comprising a quadrangular plastic support with a first face and a second face, the first face comprises three screen-printed electrodes, in the active area of the working electrode is deposited a layer of platinum and a solution containing the enzyme lactate oxidase, which upon oxidation produces pyruvate and hydrogen peroxide, and a potentiostat is fixed to the second side. Measurement method with the aforementioned device comprising the following steps: deposition of sweat containing lactic acid in concentration between 4 mm and 40 mm in the active area of the working electrode; application by the potentiostat of a potential between 0.4 v and 0.9 v between the working electrode and the reference electrode; reading of the generated current between 2 ma and 20 ma by the potentiostat. (Machine-translation by Google Translate, not legally binding)
    公开号:ES2638737A1
    申请号:ES201630518
    申请日:2016-04-22
    公开日:2017-10-23
    发明作者:Julia ARCOS MARTÍNEZ；Hugo Silva；Javier Sedano Franco；Alberto NAVARRO BENITO；Miguel PORTA GARCÍA
    申请人:Universidad de Burgos；
    IPC主号:
    专利说明:

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    Sample to analyze is usually a drop whose contour when deposited on a surface is circular. It is also advantageous that the active area (1.22) of the counter electrode (1.13) consists of a straight section (1.23) that connects with an arc section (1.24), as this achieves a large area in the available space.
    For its part, as shown in Figure 3, the potentiostat (2) is arranged on an integrated circuit board (4) (“PCB”), on which a power supply is also provided ( 5) connected to it to provide electric power as well as to a digital analog converter (6), a microcontroller (7) and a communication interface (8), the three electrodes (1.11,1.12,1.13) being electrically connected to the potentiostat ( 2), this to the digital analog converter (6), this to the microcontroller (7) and this to the communication interface (8), as shown in the scheme in Figure 2.
    The potentiostat (2) thus arranged fulfills the following functions: -provides sufficient current to circulate between the working electrode (1.12) and the reference electrode (1.11); - stabilizes the reference voltage, between 0.4 V and 0.9 V, preferably 0.6 V, between said electrodes (1.11,1.12) avoiding the polarization of the reference electrode (1.11) so that no current flows through it and Therefore, the voltage applied to it is constant so that the current measurements are reliable; -provides the rest of the components with a real-time measurement for constant monitoring of the reference voltage, so that its correct operation is verified; -provides a high impedance isolation of the potentiostat (2) with the parts that connect it to the other components, specifically, the reference voltage output to be measured and the bias voltage that must be provided to the working electrode (1.12 ).
    Specifically, the potentiostat (2) can comprise: - a first operational amplifier that provides voltage between 0.4 V and 0.9 V, with a gain of 100 dB in open loop, a bandwidth of 7 MHz, an effect nonlinear 5 V / µs, a rejection of the common current mode of 90 dB; -a second operational amplifier that in turn combines two amplifiers, third and fourth, in a surface-mounted encapsulation that isolates the impedance of voltage input to the working electrode (1.12) and the reference electrode (1.11) and output electrode their current, with a bandwidth of 1 MHz, a non-linear effect of 0.6 V / µs, an input bias current of 1 pA at 25 ° C and an input impedance of 100 MOhm.
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    Optionally, the potentiostat (2) is also connected to a Butterworth filter
    (9) third order low pass with Sallen-Key structure and unit gain.
    Advantageously, it is verified that one option is that the microcontroller (7) has 8-bit storage load architecture.
    The main functions of the microcontroller (7) are as follows: -load the preprogrammed parameters or program the parameters for the potentiostat (2) and for the digital analog converter (6) for calculating the signal obtained from the working electrode (1.12) and of the reference electrode (1.11); - monitors in real time the voltage of the reference electrode (1.11) to check the correct operation of the potentiostat (2); -control the on and off of the potentiostat (2); - acquires the values of the working electrode signal from the digital analog converter (6)
    (1.12) and of the reference electrode (1.11); -applies programmed algorithms for the conversion and interpretation of the values obtained from the signal and supply voltage of the working electrode (1.12) and the reference electrode (1.11); -manage communications with the communication interface (8).
    Optionally, the integrated circuit board (4) and all the components that are arranged on it (2,5,6,7,8,9) are arranged inside an aluminum enclosure (10) for protection against electromagnetic interference and noises
    The measurement procedure for the detection of lactic acid with an electrode device as described comprises the following steps: sweat deposition containing lactic acid in concentration between 3 mM and 50 mM in the active area (1.21) of the working electrode (1.12); -application by the potentiostat (2) of a potential between 0.4 V and 0.9 V between the working electrode (1.12) and the reference electrode (1.11); - reading of the current generated between 2 mA and 20 mA by the potentiostat (2).
    After reading the generated current, the following steps take place: -transmission of said reading to the digital analog converter (6) to convert the analog signal into digital;
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    权利要求:
    Claims (1)
    [1]
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    同族专利:
    公开号 | 公开日
    ES2638737B1|2018-08-14|
    引用文献:
    公开号 | 申请日 | 公开日 | 申请人 | 专利标题
    ES1062351U|2006-02-28|2006-06-16|Universidad De Burgos|Electrodic system disposable for electrochemical analysis "in situ" |
    ES2484665A1|2013-02-11|2014-08-11|Universidad De Burgos|Electrode device for the detection of gluconic acid, method of manufacturing and use of said device |
    ES2524991A1|2013-06-12|2014-12-16|Universidad De Burgos|Electrode device for the detection of sulfites, method of manufacturing and use of said device |
    WO2016050226A1|2014-09-30|2016-04-07|Vysoke Uceni Technicke V Brne|Potentiostat|
    法律状态:
    2018-08-14| FG2A| Definitive protection|Ref document number: 2638737 Country of ref document: ES Kind code of ref document: B1 Effective date: 20180814 |
    优先权:
    申请号 | 申请日 | 专利标题
    ES201630518A|ES2638737B1|2016-04-22|2016-04-22|Electrode device and measurement procedure for the detection of lactic acid|ES201630518A| ES2638737B1|2016-04-22|2016-04-22|Electrode device and measurement procedure for the detection of lactic acid|
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